We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Tag
C-terminal 10xHis-tagged
Molecular Weight
53.9 kDa
Relevance
Alkaline phosphatase that can hydrolyze various phosphate compounds.
Source
Mammalian cell
Form
Lyophilized powder
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Lyophilized from a 0.2 um filtered PBS, 6% Trehalose, pH 7.4
Purity
Greater than 95% as determined by SDS-PAGE.
Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. <br />Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Reference
TNAP limits TGF-beta-dependent cardiac and skeletal muscle fibrosis by inactivating the SMAD2/3 transcription factors.<br />Arno B., Galli F., Roostalu U., Aldeiri B.M., Miyake T., Albertini A., Bragg L., Prehar S., McDermott J.C., Cossu G.<br />J Cell Sci 132:jcs234948-jcs234948 (2019)