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Home / Proteins & Peptide

Recombinant Moloney Mouse Leukemia Virus Reverse Trancscriptase

SKU : BHP11001425

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ProSpec-Tany TechnoGene Ltd.

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Mfr.No.	enz-310
Description	MMLV (Moloney Murine Leukemia Virus) Reverse Transcriptase is a DNA polymerase that synthesizes a complementary DNA strands from single-stranded RNA, DNA, or an RNA-DNA hybrid as a template. This recombinant enzyme was purified from E.coli, which carried a modified MMLV-RT gene. Compared to AMV Reverse Transcriptase, this enzyme has a much weaker 5' - 3' ribonuclease H activity, which allows the syntesis of longer cDNAs (>7kb).
Regulatory	RUO
Source	Recombinant E. coli strain
Nature	Recombinant

Antigen	MMLV RT

Shipping	Shipped with Ice Packs
Buffer	5X Reaction Buffer: 250mM Tris-HCl (pH8.3), 375mM KCl, 15mM MgCl2, and 50mM DTT.
Description	Formulation: 50mM Tris-HCl, 0.1M NaCl, 0.1% Triton X-100, 2mM DTT, 0.1mM EDTA and 50% glycerol. Unit Definition: One unit is defined as the amount of enzyme required to catalyze the incorporation of 1nmol of deoxyribonucleotide into acid-insoluble forms in 10 minutes at 37oC, using poly(A)-oligo(dT)12-18 as the template-primer. Standard cDNA Synthesis Conditions50mM Tris-HCl (pH8.3), 75mM KCl, 3mM MgCl2, 10mM DTT, 1.0mM each dATP, dGTP, dCTP, and dTTP, 0.2 mg radom hexamer,1-5mg RNA, 200units M-MLV RT. The reaction volume was 20ml and the incubation was 45 min at 42oC.
Format	Sterile Filtered clear solution (200 U/µl).
Expiry Date	Stable for 5 days at 10°C, for longer period of time store at -20°C. Please prevent freeze-thaw cycles.

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SKU	Supplier No.	Size	Your price	Quantity
BHP11001425	enz-310	2	$60		Add to Cart
BHP11001425	enz-310		$60		Add to Cart
BHP11001425	enz-310	10	$60		Add to Cart
BHP11001425	enz-310		$145		Add to Cart
BHP11001425	enz-310	50	$60		Add to Cart
BHP11001425	enz-310		$600		Add to Cart

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