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Home / Proteins & Peptide

Recombinant IL-12, Human(HEK 293-expressed)

SKU : BHP11300271

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Mfr.No.	BK0312‐1mg
Description	Interleukin 12 (IL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine originally identified in the medium of activated human B lymphoblastoid cell lines. The p40 subunit of IL-12 has been shown to have extensive amino acid sequence homology to the extracellular domain of the human IL-6 receptor while the p35 subunit shows distant but significant sequence similarity to IL-6, G-CSF, and chicken MGF. These observations have led to the suggestion that IL-12 might have evolved from a cytokine/soluble receptor complex. Human and mouse IL-12 share 70% and 60% amino acid sequence homology in their p40 and p35 subunits, respectively. IL-12 apparently shows species specificity with human IL-12 reportedly showing minimal activity in the mouse system.
Alternative Names	Interleukin-12 ; IL12
Regulatory	RUO
Source	HEK 293
Host	Human
Endotoxin Level	< 0.2 EU/μg, determined by LAL method.
Biological Activity Comment	EC50 < 2 ng/ml, measured by the induction of IFN-γ from NK cells co-stimulated with IL-18, corresponding to a specific activity of > 1×10ˆ6 units/mg.
Nature	Recombinant

Weight	Approximately 75 kDa, consisting of a 306 amino acid residue p40 subunit and a 197 amino acid residue p35 subunit.
Antigen	IL-12

Description	Reconstituted in ddH2O or PBS at 100 μg/ml.
Format	Lyophilized after extensive dialysis against PBS. Sterile Filtered White lyophilized (freeze-dried) powder.
Purity	> 95% as analyzed by SDS-PAGE and HPLC.
Storage	Lyophilized recombinant Human Interleukin 12(IL-12) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rh-IL12 should be stable up to 1 week at 4°C or up to 2 months at -20°C.

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SKU	Supplier No.	Size	Your price	Quantity
BHP11300271	BK0312‐1mg	1mg	$4686.67		Add to Cart
BHP11300271	BK0312‐1mg	25μg	$423.33		Add to Cart
BHP11300271	BK0312‐1mg	5μg	$188.67		Add to Cart

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