Please refer to the printed manual for detailed information.
Background
UBE2F is a member of the ubiquitin-conjugating E2 family whose members perform the second step in the ubiquitination reaction. Initially identified as the main process for protein degradation, ubiquitination is believed nowadays to be crucial for a wider range of cellular processes. The outcome of the ubiquitin-conjugation reaction, and thereby the fate of the substrate, is heavily dependent on the number of ubiquitin molecules attached and how these ubiquitin molecules are inter-connected. To deal with this complexity and to allow adequate ubiquitination in time and space, a highly sophisticated conjugation machinery has been developed. In a sequential manner, ubiquitin becomes activated by an ubiquitin-activating enzyme (E1), which then transfers the ubiquitin to a group of ubiquitin-conjugating enzymes (E2s). Next, ubiquitin-loaded E2s are interacting with ubiquitin protein ligases (E3s) and ubiquitin is conjugated to substrates on recruitment by the E3. These three key enzymes are operating in a hierarchical system, wherein two E1s and 35 E2s have been found and hundreds of E3s have been identified in humans.
Reactivity
Human
Tag
N-His
Description
A DNA sequence encoding the human UBE2F isoform 1 (Q969M7-1) (Met 1-Arg 185) was expressed, with a polyhistide tag at the N-terminus.
Formulation
Lyophilized from sterile PBS, 10% glycerol, 2mM DTT, pH 7.5
Host
E.coli
Accession Number
Q969M7-1
Endotoxin Level
Please contact us for more information.
Mol Weight
22.9 kDa
Mw(kda)
22.9 kDa
Sequence Info
Met 1-Arg 185
Shipping
This product is provided as lyophilized powder which is shipped with ice packs.
Stotage Note
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.