Synaptosomal-associated protein 25, also known as Super protein, Synaptosomal-associated 25 kDa protein, SNAP25 and SNAP, is a cytoplasm and cell membrane protein which belongs to the SNAP-25 family. SNAP25/ SUP contains 2 t-SNARE coiled-coil homology domains. SNAP25/ SUP is a membrane bound protein anchored to the cytosolic face of membranes via palmitoyl side chains in the middle of the molecule. SNAP25/ SUP protein is a component of the SNARE complex, which is proposed to account for the specificity of membrane fusion and to directly execute fusion by forming a tight complex that brings the synaptic vesicle and plasma membranes together. SNAP25/ SUP is a Q-SNARE protein contributing two α-helices in the formation of the exocytotic fusion complex in neurons where it assembles with syntaxin-1 and synaptobrevin. SNAP25/ SUP is involved in the molecular regulation of neurotransmitter release. It may play an important role in the synaptic function of specific neuronal systems. SNAP25/ SUP associates with proteins involved in vesicle docking and membrane fusion. SNAP25/ SUP regulates plasma membrane recycling through its interaction with CENPF. SNAP25/ SUP inhibits P/Q- and L-type voltage-gated calcium channels located presynaptically and interacts with the synaptotagmin C2B domain in Ca2+-independent fashion. In glutamatergic synapses SNAP25/ SUP decreases the Ca2+ responsiveness, while it is naturally absent in GABAergic synapses.
Tag
His tag
Description
A DNA sequence encoding the human SNAP25 (P60880-1) (Met 1-Gly 206) was expressed, with a polyhistide tag at the N-terminus.
Regulatory
RUO
Host
E. coli
Nature
Recombinant
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Buffer
Lyophilized from sterile PBS, pH 7.4
Description
Please refer to the printed manual for detailed information.
Purity
> 90 % as determined by SDS-PAGE
Expiry Date
Samples are stable for up to twelve months from date of receipt at -70℃
Immunogen Species
Homo Sapiens (Human)
Attachment
Reviews of Recombinant Human SNAP25/SUP Protein (His Tag)