Please refer to the printed manual for detailed information.
Background
Sialate O-acetylesterase belongs to the family of hydrolases, specifically those acting on carboxylic ester bonds. It is widely expressed with high expression in the testis, prostate, and colon. The systematic name of this enzyme class is N-acyl-O-acetylneuraminate O-acetylhydrolase. Other names in common use include N-acetylneuraminate acetyltransferase, sialate 9(4)-O-acetylesterase, and sialidase. Sialate O-acetylesterase catalyzes the removal of O-acetyl ester groups from position 9 of the parent sialic acid, N-acetylneuraminic acid. Defects in Sialate O-acetylesterase are a cause of autoimmune disease type 6 (AIS6). Individuals manifesting susceptibility to autoimmune disease type 6 can suffer from juvenile idiopathic arthritis, rheumatoid arthritis, multiple sclerosis, Sjogren syndrome, systemic lupus erythematosus, type 1 diabetes, ulcerative colitis, and Crohn disease.
Reactivity
Human
Tag
C-His
Description
A DNA sequence encoding the human SIAE (Met 1-Lys523) (Q9HAT2-1) was expressed, with a C-terminal polyhistidine tag.
Formulation
Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0
Host
Baculovirus-Insect Cells
Accession Number
Q9HAT2-1
Endotoxin Level
< 1.0 EU per ug as determined by the LAL method.
Mol Weight
57.4 kDa
Mw(kda)
57.4 kDa
Sequence Info
Met 1-Lys523
Shipping
This product is provided as lyophilized powder which is shipped with ice packs.
Stotage Note
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.