Please refer to the printed manual for detailed information.
Background
Tyrosine-protein phosphatase non-receptor type 2; also known as T-cell protein-tyrosine phosphatase; PTPN2 and PTPT; is a cytoplasm protein which belongs to the protein-tyrosine phosphatase family and Non-receptor class 1 subfamily. Members of the protein tyrosine phosphatase ( PTP ) family share a highly conserved catalytic motif; which is essential for the catalytic activity. TC-PTP / PTPN2 is a cytosolic tyrosine phosphatase that functions as a negative regulator of a variety of tyrosine kinases and other signaling proteins. The expression of TC-PTP / PTPN2 plays a role of tumor suppressor and may modulate response to treatment. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth; differentiation; mitotic cycle; and oncogenic transformation. Epidermal growth factor receptor and the adaptor protein Shc were reported to be substrates of this PTP; which suggested the roles in growth factor mediated cell signaling. TC-PTP / PTPN2 is an enzyme that is essential for the proper functioning of the immune system and that participates in the control of cell proliferation; and inflammation. TC-PTP / PTPN2 was identified as a negative regulator of NUP214-ABL1 kinase activity.
Reactivity
Human
Tag
N-His & GST
Description
A DNA sequence encoding the amino acid (Met 1-Asn 314) of human PTPN2 (P17706-1) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Formulation
Lyophilized from sterile PBS; pH 7.4
Host
Baculovirus-Insect Cells
Accession Number
P17706-1
Endotoxin Level
< 1.0 EU per ug as determined by the LAL method.
Bioactivity
Measured by its ability to dephosphorylate a phosphotyrosine residue in an EGF receptor 988-998 phosphopeptide substrate; R&D Systems; Catalog # ES006.The specific activity is > 30 nmoles/min/ug.
Mol Weight
64.4 kDa
Mw(kda)
64.4 kDa
Sequence Info
Met 1-Asn 314
Shipping
This product is provided as lyophilized powder which is shipped with ice packs.
Stotage Note
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.