Serine/ threonine-protein kinase PLK1/ PLK-1, also known as polo-like kinase 1 (PLK-1) or serine/ threonine-protein kinase 13 (STPK13), Polo-like kinases (PLKs), is a family of four serine/ threonine protein kinases that are critical regulators of cell cycle progression, mitosis, cytokinesis, and the DNA damage response. PLK1/ PLK-1 is ubiquitously expressed. The mRNA and protein expression of PLK1/ PLK-1, -2 and -4 are coordinately regulated during cell cycle progression, but PLK3 levels are independent of the other three family members. PLK1/ PLK-1 is the most well characterized member of this family and strongly promotes the progression of cells through mitosis. During the various stages of mitosis PLK1/ PLK-1 localizes to the centrosomes, kinetochores and central spindle. PLKs are dysregulated in a variety of human cancers. PLK1/ PLK-1 overexpression correlates with cellular proliferation and poor prognosis. Serine/ threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/ C inhibitors, and the regulation of mitotic exit and cytokinesis. It is required for recovery after DNA damage checkpoint and entry into mitosis. PLK1/ PLK-1 is required for kinetochore localization of BUB1B, spindle pole localization of isoform 3 of SGOL1 and plays a role in regulating its centriole cohesion function. PLK1/ PLK-1 Phosphorylates BORA, and thereby promotes the degradation of BORA. PLK1/ PLK-1 also contributes to the regulation of AURKA function and phosphorylates SGOL1.
Tag
His tag
Description
A DNA sequence encoding the human PLK1 (NP_005021.2) (Met 1-Ser 603) was expressed, with a polyhistidine tag at the N-terminus.
Regulatory
RUO
Host
Baculovirus-Insect Cells
Endotoxin Level
< 1.0 EU per μg of the protein as determined by the LAL method
Nature
Recombinant
Shipping
Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.Shipment made at ambient temperature may seriously affect the activity of the ordered products.