Nodal, also known as Nodal homolog, is a secreted protein member of the Transforming Growth Factor-beta (TGF-beta) superfamily. It is essential for the formation of mesoderm and axial patterning during early vertebrate embryonic development.
Alternative Names
Nodal homolog
Quantity
25 µg
Regulatory
RUO
Source
E. coli
Host
Human
Endotoxin Level
<1.0 EU/μg of recombinant protein as determined by the LAL method
Biological Activity Comment
100 ng/mL phosphorylation activity as determined by its ability to induce Smad2 phosphorylation in P19 mouse embryonal carcinoma cells
Weight
13.1 kDa
Description
Reconstitute in sterile 4mM HCl at 100µg/mL (containing at least 0.1% human or bovine serum albumin)
Format
Lyophilized PowderLyophilized from a 0.2 µm filtered solution in Acetonitrile and TFA (with BSA as a carrier protein)
Purity
>95% as determined by SDS-PAGE
Storage
The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity.