Please refer to the printed manual for detailed information.
Background
Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A, also known as Alpha-mannoside beta-1,6-N-acetylglucosaminyl-transferase, Mannoside acetylglucosaminyltransferase 5, N-acetylglucosaminyl-transferase V, MGAT5 and GGNT5, is a single-pass type I I membrane protein which belongs to the glycosyltransferase 18 family. MGAT5 / GGNT5 catalyzes the addition of N-acetylglucosamine in beta 1-6 linkage to the alpha-linked mannose of biantennary N-linked oligosaccharides. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides. The central nervous system (CNS) is rich in glycoconjugates, located on cell surface and in extracellular matrix. MGAT5 / GGNT5 modification of complex-type N-glycans on CNS glycoproteins is involved in the regulation of depression-like behavior. Inhibitors of MGAT5 / GGNT5 might be useful in the treatment of malignancies by targeting their dependency on focal adhesion signaling for growth and metastasis.Immune Checkpoint Immunotherapy Cancer Immunotherapy Targeted Therapy
Reactivity
Human
Tag
C-His
Description
A DNA sequence encoding the human MGAT5 (NP_002401.1) C-terminal segment (Leu 189-Leu 741) was fused with a polyhistidine tag at the C-terminus and a signal peptide at the terminus.
Formulation
Lyophilized from sterile PBS, pH 7.4
Host
HEK293 Cells
Accession Number
NP_002401.1
Endotoxin Level
< 1.0 EU per ug as determined by the LAL method.
Bioactivity
Measured by its ability to transfer N-Acetyl-alpha-D-glucosamine from UDP-N-Acetyl-alpha-D-glucosamine to a biantennary N-linked core pentasaccharide in a CD39L3 coupled assay. The specific activity is >10pmoles/min/ug
Mol Weight
65 kDa
Mw(kda)
65 kDa
Sequence Info
Leu 189-Leu 741
Shipping
This product is provided as lyophilized powder which is shipped with ice packs.
Stotage Note
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.