Please refer to the printed manual for detailed information.
Background
GPR114 belongs to the G-protein coupled receptor 2 family. Members of this family share a common molecular architecture which consists of seven transmembrane domains, three extracellular loops, three intracellular loops, an amino-terminal extracellular domain and an intracellular carboxyl terminus. It is thought that light acts as the activating stimulus of a G-protein-coupled receptor (GPCR). GPCRs are expected to have molecular function (G-protein coupled receptor activity) and to localize in various compartments (endoplasmic reticulum membrane, plasma membrane, integral to membrane). Family B of the GPCRs is a small but structurally and functionally diverse group of proteins that includes receptors for polypeptide hormones, molecules thought to mediate intercellular interactions at the plasma membrane and a group of Drosophila proteins that regulate stress responses and longevity. GPR114 contains 1 GPS domain. GPR114 gene has been proposed to participate in processes (G-protein coupled receptor protein signaling pathway, neuropeptide signaling pathway).
Reactivity
Human
Tag
C-His
Description
A DNA sequence encoding the human HPX (NP_000604.1) (Met 1-His 462) with a C-terminal polyhistidine tag was expressed.
Formulation
Lyophilized from sterile PBS, pH 7.4
Host
HEK293 Cells
Accession Number
NP_000604.1
Endotoxin Level
< 1.0 EU per ug of the protein as determined by the LAL method.
Bioactivity
Measured by its ability to bind protoporphyrin IX (PPPIX). Recombinant human Hemopexin binds > 10 uM PPPIX, resulting in a 50% decrease in the fluorescence signal of human Hemopexin.
Mol Weight
50.7 kDa
Mw(kda)
50.7 kDa
Sequence Info
Met 1-His 462
Shipping
This product is provided as lyophilized powder which is shipped with ice packs.
Stotage Note
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.