Flt3-Ligand Human Recombinant produced in HEK293 cells is a glycosylated, polypeptide chain, migrates as a diffuse band on SDS-PAGE due to heterogeneous glycosylation at 30kDa. Flt3-Ligand is purified by proprietary chromatographic techniques.
The ED50 as determined by the dose dependent proliferation of human OCI-AML5 cells is 0.5 to 1.0 ng/ml (Specific Activity: 2.0 x 1,000,000 – 1.0 x 1,000,000 units/mg).
Nature
Recombinant
Uniprot
P49771
Description
Formulation: The protein was lyophilized with no additives. Solubility: It is recommended to reconstitute the lyophilized Flt3-Ligand in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Format
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. ; ; ; (b) Analysis by SDS-PAGE.