FGF-8 is a heparin binding protein of the FGF family which works to control cell division, differentiation, and function. These proteins are especially prominent during prenatal development and postnatal growth. FGF-8 can be spliced in four different ways. The products of the FGF-8 splicing events are as follows: FGF8A, 8B, 8E and 8F. This recombinant product represents isoform 8B and is expressed in human 293 cells as a monomeric glycoprotein with a molecular mass of 30 to 45 kDa. This wide molecular mass is due to glycosylation, which is absent when this cytokine is expressed in E. coli.
Quantity
100 µg
Regulatory
RUO
Source
HEK293
Host
Human
Endotoxin Level
<1 EU/µg of recombinant protein as determined by the LAL method
Biological Activity Comment
The EC(50) as determined by dose-dependent stimulation of the proliferation of the Balb/3T3 cell line was found to be ≤ 60 ng/mL.
Weight
30 to 45 kDa, monomer, glycosylated
Description
A quick spin of the vial followed by reconstitution in sterile water containing 0.1% endotoxin-free recombinant human serum albumin (HSA).
Format
Lyophilized Powder10 mM Tris-HCl pH 7.4 + 800 mM NaCl
Purity
>95% as determined by SDS-PAGE
Storage
The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months. Avoid repeated freeze/thaw cycles.