A DNA sequence encoding the human CANT1 (Q8WVQ1-1) (Gly80-Ile401) was expressed;with the fused Fc region of human IgG1 at the N-terminus.
Alternative Names
DBQD;SCAN-1;SCAN1;SHAPY
Reconstitution
Please refer to the printed manual for detailed information.
Background
CANT1(calcium activated nucleotidase 1) belongs to the apyrase family. Apyrase is a calcium-activated plasma membrane-bound enzyme (magnesium can also activate it) (EC 3.6.1.5) that catalyses the hydrolysis of ATP to yield AMP and inorganic phosphate. Two isoenzymes are found in commercial preparations from S. tuberosum. One with a higher ratio of substrate selectivity for ATP: ADP and another with no selectivity. It can also act on ADP and other nucleoside triphosphates and diphosphates with the general reaction being NTP -> NDP + Pi -> NMP + 2Pi. The salivary apyrases of blood-feeding arthropods are nucleotide hydrolysing enzymes are implicated in the inhibition of host platelet aggregation through the hydrolysis of extracellular adenosine diphosphate. CANT1 functions as a calcium-dependent nucleotidase with a preference for UDP. Defects in CANT1 are the cause of desbuquois dysplasia.
Reactivity
Human
Tag
N-Fc
Formulation
Lyophilized from sterile PBS; pH 7.4
Host
HEK293 Cells
Accession Number
Q8WVQ1-1
Endotoxin Level
< 1.0 EU per ug as determined by the LAL method.
Mol Weight
64.3 kDa
Mw(kda)
64.3 kDa
Sequence Info
Gly80-Ile401
Shipping
This product is provided as lyophilized powder which is shipped with ice packs.
Stotage Note
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.