Amphiregulin is a trans-membrane protein whose protease mediated digestion of the extracellular domain enables release of mature AR. Amphiregulin works to stimulate the proliferation of some animal keratinocyles, epithelial cells and fibroblasts. It has been shown that the mRNA expression of amphiregulin can be detected in numerous carcinoma cell lines and the epithelial cells of various human tissues, including colon, stomach, breast, ovary and kidney.
Alternative Names
AR ; Colorectum cell-derived growth factor ; CRDGF Recombinant Human Amphiregulin (AREG)
Appearance
Lyophilized Powder
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein
Molecular Weight
11.5 kDa
Quantity
1.0 mg
Source
E. coli
Usage
For research use only. Not for diagnostic or therapeutic use.
Accession Number
P15514
Endotoxin Level
<1.0 EU/μg of recombinant protein as determined by the LAL method.
Bioactivity
Measured in a cell proliferation assay using mouse embryonic fibroblast 3T3 cells. The ED(50) for this effect is <0.5 ng/mL.,
Gel Image
Gene Id
374
Gene Name
AREG
Purity
>95% as determined by SDS-PAGE
Storage
The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity.Avoid repeated freeze/thaw cycles.