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Recombinant Bacillus subtilis D-alanine--D-alanyl carrier protein ligase(dltA)

SKU : BHP10505669

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CUSABIO TECHONOLOGY LLC

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Mfr.No.	CSB-EP336594BRJ(A4)
Description	Catalyzes the first step in the D-alanylation of lipoteichoic acid (LTA), the activation of D-alanine and its transfer onto the D-alanyl carrier protein (Dcp) DltC. In an ATP-dependent two-step reaction, forms a high energy D-alanyl-AMP intermediate, followed by transfer of the D-alanyl residue as a thiol ester to the phosphopantheinyl prosthetic group of the Dcp. D-alanylation of LTA plays an important role in modulating the properties of the cell wall in Gram-positive bacteria, influencing the net charge of the cell wall.
Alternative Names	D-alanine--poly(phosphoribitol) ligase subunit 1 D-alanine-activating enzyme dae
Tag	N-terminal 6xHis-tagged
Regulatory	RUO
Relevance	Catalyzes the first step in the D-alanylation of lipoteichoic acid (LTA), the activation of D-alanine and its transfer onto the D-alanyl carrier protein (Dcp) DltC. In an ATP-dependent two-step reaction, forms a high energy D-alanyl-AMP intermediate, followed by transfer of the D-alanyl residue as a thiol ester to the phosphopantheinyl prosthetic group of the Dcp. D-alanylation of LTA plays an important role in modulating the properties of the cell wall in Gram-positive bacteria, influencing the net charge of the cell wall.
Source	E.coli
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Protein Name	D-alanine--poly(phosphoribitol) ligase subunit 1D-alanine-activating enzymedae
Expression Region	1-503aa
Mol Weight	59.8 kDa
Nature	Recombinant
Protein Description	Full Length

Subcellular Location	Cytoplasm
Uniprot	P39581, P39581
Database Links	KEGG:bsu:BSU38500 STRING:224308.Bsubs1_010100020786
Function	Catalyzes the first step in the D-alanylation of lipoteichoic acid (LTA), the activation of D-alanine and its transfer onto the D-alanyl carrier protein (Dcp) DltC. In an ATP-dependent two-step reaction, forms a high energy D-alanyl-AMP intermediate, followed by transfer of the D-alanyl residue as a thiol ester to the phosphopantheinyl prosthetic group of the Dcp. D-alanylation of LTA plays an important role in modulating the properties of the cell wall in Gram-positive bacteria, influencing the net charge of the cell wall.
Protein Families	ATP-dependent AMP-binding enzyme family, DltA subfamily
Research Area	Cancer
Weight	59.8 kDa

Stotage Note	Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Buffer	Tris-based buffer，50% glycerol
Purity	Greater than 85% as determined by SDS-PAGE.
Storage	The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
Storage Buffer	Tris-based buffer,50% glycerol

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SKU	Supplier No.	Size	Your price	Quantity
BHP10505669	CSB-EP336594BRJ(A4)	1mg	$2466		Add to Cart
BHP10505669	CSB-EP336594BRJ(A4)	100ug	$729		Add to Cart
BHP10505669	CSB-EP336594BRJ(A4)	20ug	$388		Add to Cart

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