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Home / Proteins & Peptide

Recombinant Active Glycerol kinase Protein (His Tag)

SKU : BHP10306244

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Elabscience Biotechnology Co.,Ltd

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Mfr.No.	PKSQ500004
Description	A DNA sequence encoding the Escherichia coli Glycerol kinase (2T-502E) was expressed with a polyhistidine tag at the N-terminus and C-terminus.
Alternative Names	GK, glpK
Reconstitution	Please refer to the printed manual for detailed information.
Background	Glycerol kinase from E. coli (glpK) catalyzes the ATP-dependent phosphorylation of glycerol to produce sn-glycerol-3-phosphate (G3P), the first and rate-limiting step in the utilization of glycerol. In the presence of glycerol, glpK is stimulated by interaction with the membrane-bound glycerol facilitator. In the presence of glucose, glpK activity is allosterically inhibited by fructose-1,6-bisphosphate (FBP) of the glycolytic pathway. Under physiological conditions, the enzyme is in an equilibrium between the active dimer and the inactive tetramer. FBP binds to and stabilizes the inactive form, therefore shifting the usage of glycerol metabolic pathway to glycolytic pathway. GlpK is a member of a superfamily of ATPases that includes actin, hexokinase and the heat shock protein hsc70. Although these proteins are dissimilar in amino acid sequence and function, they share similar tertiary folds and likely the same catalytic mechanism. The enzyme activity was measured using a phosphatase-coupled kinase assay.
Reactivity	Escherichia coli (strain K12)
Tag	N/C-His
Formulation	Lyophilized from sterile potassium phosphate, pH 7.4
Host	E.coli
Accession Number	P0A6F3
Endotoxin Level	Not tested.
Bioactivity	Measured by its ability to transfer phosphate from ATP to glycerol. The specific activity is ? 200U/mg protein, as measured under the described conditions.
Mol Weight	56.1kDa
Mw(kda)	56.1kDa

Sequence Info	2T-502E

Shipping	This product is provided as lyophilized powder which is shipped with ice packs.
Stotage Note	Samples are stable for up to twelve months from date of receipt at -70°C.Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Purity	>90 % as determined by reducing SDS-PAGE.

Product Manual	Data Sheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP10306244	PKSQ500004	100ug	$499		Add to Cart
BHP10306244	PKSQ500004	400ug	$999		Add to Cart

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