A DNA sequence encoding the 2019-nCoV spike protein S1 Subunit (YP_009724390.1) (Val16-Arg685) was expressed with the Fc region of human IgG1 at the C-terminus.
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Reactivity
Virus
Tag
C-Fc
Formulation
LyopHilized from sterile PBS, pH 7.4.
Host
HEK293 Cells
Accession Number
YP_009724390.1
Endotoxin Level
< 1.0 EU per ug as determined by the LAL method.
Bioactivity
Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (His tag) (Cat#PKSR030508) at 2ug/mL (100uL/well) can bind Recombinant 2019-nCoV S1 Protein (Fc Tag)(PKSR030502), the EC50 of SARS-CoV-2 (2019-nCoV) Spike Protein (S1 Subunit, Fc Tag) is 25-60 ng/mL.Immobilized human ACE2 protein (His tag) at 2ug/mL (100uL/well) can bind Recombinant 2019-nCoV S1 Protein (Fc Tag) (PKSR030502), the EC50 of Recombinant 2019-nCoV S1 Protein (Fc Tag) is 25-60 ng/mL.
Mol Weight
101.7 kDa
Mw(kda)
101.7 kDa
Sequence Info
Val16-Arg685
Shipping
This product is provided as lyophilized powder which is shipped with ice packs.
Stotage Note
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Purity
> 85 % as determined by reducing SDS-PAGE.
Reviews of Recombinant 2019-nCoV S1 Protein (Fc Tag)