Recombinant 2019-nCoV Papain-Like Protease

Mfr.No.	EPT114
Description	Recombinant 2019-nCoV Papain-like Protease is produced by our E.coli expression system and the target gene encoding Glu1564-Lys1878 is expressed.
Alternative Names	Papain-like Protease; PLpro; PL-PRO; pp1a; Replicase polyprotein 1a
Background	Replication of severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) requires proteolytic processing of the replicase polyprotein by two viral cysteine proteases, a chymotrypsin-like protease (3CLpro) and a papain-like protease (PLpro). These proteases are important targets for development of antiviral drugs that would inhibit viral replication and reduce mortality associated with outbreaks of SARS-CoV. PLpro is a cysteine protease located within the non-structural protein 3 (NS3) section of the viral polypeptide. PLPro activity is required to process the viral polyprotein into functional, mature subunits; specifically, PLPro cleaves a site at the amino-terminus of the viral replicase region. In addition to its role in viral protein maturation, PLPro possesses a deubiquitinating and deISGylating activity. In vivo, this protease antagonizes innate immunity by inhibiting IRF3-induced production of type I interferons.
Biological Activity	Measured by its ability to convert the fluorogenic peptide substrate, Arg-Leu-Arg-Gly-Gly-AMC (RLRGGAMC) to RLRGG and 7-amino-4-methylcoumarin (AMC). The Specific Activity is 241.7 pmol/min/ug.
Molecular Weight	35.8 KDa
Accession Number	QHD43415.1

Shipping	The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below.
Purity	Greater than 95%
Storage	Store at <=-70°C, stable for 6 months after receipt. Store at <=-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.

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SKU	Supplier No.	Size	Your price	Quantity
BHP15200114	EPT114	50ug	$839		Add to Cart