Please refer to the printed manual for detailed information.
Background
The coronaviral proteases, papain-like protease (PLpro) and 3C-like protease (3CLpro), are attractive antiviral drug targets because they are essential for coronaviral replication. PLpro has the additional function of stripping ubiquitin and ISG15 from host-cell proteins to aid coronaviruses in their evasion of the host innate immune responses. Targeting PLpro with antiviral drugs may have an advantage in not only inhibiting viral replication but also inhibiting the dysregulation of signaling cascades in infected cells that may lead to cell death in surrounding, uninfected cells.
Reactivity
virus
Tag
N-6His
Description
A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) papain-like protease (YP_009725299.1) (Glu1564-Val1880) was expressed with a polyhistidine tag at the N-terminus.
Formulation
Lyophilized from sterile 20 mM Tris 500mM NaCl, pH 7.4.
Host
E.coli
Accession Number
YP_009725299.1
Endotoxin Level
< 1.0 EU per ug as determined by the LAL method.
Bioactivity
Measured by its ability to cleave a fluorogenic peptide substrate, Arg-Leu-Arg-Gly-Gly-AMC (RLRGGAMC).The Specific Activity is >80pmols/min/ug
Mol Weight
36.79 kDa
Mw(kda)
36.79 kDa
Sequence Info
Glu1564-Val1880
Shipping
This product is provided as lyophilized powder which is shipped with ice packs.
Stotage Note
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.