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Home / Proteins & Peptide

Rbx1/CUL4B/DDB1/DCAF15/DDA1 Complex Recombinant

SKU : BHP18201467

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BPS Bioscience

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Mfr.No.	101497-2
Immunogen Region	Rbx1: 2-108(end); CUL4B: 3-913; DDB1: 2-1140(end); DCAF15: 2-600(end); DDA1: 2-102(end)
Tag	N-terminal FLAG-Avi-Tag, N-terminal His-Tag
Description	Recombinant Recombinant human complex of RBx1 (ring-box 1), CUL4B (cullin 4B), DBB1 (damage specific DNA binding protein 1), DCAF15 (DDB1 and CUL4 associated factor 15), and DDA1 (DET1 and DDB1 associated 1). RBx1, full length, encompassing amino acids 2-108(end). This construct contains an N-terminal His-tag (6xHis). CUL4B, encompassing amino acids 3-913(end). This construct contains an N-terminal His-tag (6xHis). DBB1, full length, encompassing amino acids 2-1140(end). This construct contains an N-terminal Flag-tag. DCAF15, full length, encompassing amino acids 2-600(end). This construct contains an N-terminal Flag-tag. DDA1, full length, encompassing amino acids 2-102(end). This construct contains an N-terminal Flag-tag. These recombinant proteins are co-expressed in a HEK293 expression system and affinity purified. host species: HEK293 Host species: HEK293
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Notes	Assay was performed according to DCAF15 TR-FRET Assay Kit with DCAF15 titrated up to 50 nM. Avoid freeze/thaw cycles.
GenBank	DDB1: NM_001923; CUL4B: NM_003588.4; DCAF15: NM_138353.4; DDA1: NM_024050.6; RBX1: NM_014248
Mw(kda)	DDB1: 128 kDa; CUL4B: 105 kDa; DCAF15: 67 kDa; DDA1: 13 kDa; RBX1: 13 kDa

Synonyms	DDBA, UV-DDB1, WHIKERS, XAP1, XPCE, XPE, XPE-BF, CUL-4B, MRXHF2, MRXS15, MRXSC, SFM2, C19orf72, C19orf58, PCIA1, BA554C12.1, RNF75, ROC1
Uniprot	DDB1: Q16531; CUL4B: Q13620; DCAF15: Q66K64; DDA1: Q9BW61; RBX1: P62877

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Purity	>=90%
Storage	At least 6 months at -80°C.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18201467	101497-2	50 ug	$654.50		Add to Cart

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