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Home / Proteins & Peptide

Rat PDE2A, His-GST-tags

SKU : BHP18200500

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BPS Bioscience

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Mfr.No.	60022
Description	Rat PDE2A (GenBank Accession No. NM_00143847), full length, with N-terminal His-GST-tag, MW=133 kDa, expressed in a Baculovirus infected Sf9 cell expression system host species: Sf9 cells Host species: Sf9 cells Specific activity: >= 2500 pmol/min/ug Unit definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.
Immunogen Region	full length
Tag	N-terminal His-GST-tags
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 4 mM glutathione, 3 mM DTT, and 20% glycerol.
Species	Rat
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	10 mM Tris-HCl,pH 7.4, 10 mM MgCl₂, 0.1 mg/ml BSA,0.05% Tween-20, 200 uM cAMP, 2.5 kU 5'-nucleotidase, andserial dilutions of PDE2A at 37°C for 20 min.Quantified by 5'- nucleotidase cleaving the5' -AMP product and releasing thephosphate group which is detected byMalachite Green Reagent. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended. If necessary, use carrier protein (BSA 0.1 - 0.5%).
GenBank	NM_001143847
Mw(kda)	133 kDa

Synonyms	PDE2A, phosphodiesterase 2A
Uniprot	Q01062

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Seybold, J., et al., Blood 105 (9), 3569- 3576 (2005). 2. Martinez, S.E., et al., Proc. Natl. Acad. Sci. U.S.A. 99 (20), 13260-13265 (2002).Application Reference(s): A yeast-based chemical screen identifies a PDE inhibitor that elevates steroidogenesis in mouse Leydig cells via PDE8 and PDE4 inhibition (2013)

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200500	60022	5 ug	$500.50		Add to Cart

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