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Home / Proteins & Peptide

Protein Quantification Kit (BCA Assay)

SKU : BHE13700005

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Abbkine Scientific Co., Ltd.

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Mfr.No.	KTD3001
Description	Abbkine Protein Quantification Kit (BCA Assay) provides a simple, rapid, detergent-compatible procedure for the detection and quantitation of total protein.
Alternative Names	Bicinchoninic acid protein
Features	Time savingmuch easier and four times faster than the classical Lowry method. Sensitivedetect down to 20 ug/mL and the minimum amount of detection protein reaches 0.4 ug. Compatibleunaffected by most ionic and nonionic detergents. Can be compatible with up to 5% SDS, 5% Triton X-100, 5% Tween-20, 60, 80 in the sample. Good linearitylinear working range from 20 to 2000 ug/mL. Excellent uniformity exhibits less protein-to-protein variation than dye-binding methods.
Calibration Range	20-2,000 ug/mL
Background	Abbkine Protein Quantification Kit (BCA Assay) is improved on the basis of BCA method, one of the commonly used protein concentration detection methods in the world. The principle of this method is that the protein reduces copper ions (Cu2+) to cuprous ions (Cu+ ) in alkaline conditions, and the generated Cu+ forms a purple complex with BCA, which has a strong absorption peak at 562 nm, and the absorbance value It is proportional to the protein content in the sample, and the protein concentration can be calculated according to the absorbance value.
Tag	BCA
Usage	1. Use a microplate reader to measure the absorbance at 562 nm, or the absorbance at other wavelengths between 540-595 nm. 2. The protein concentration determined by BCA method is not affected by chemical substances in most samples, and can be compatible with up to 5% SDS, 5% Triton X-100, 5% Tween20, 60, 80 in the sample. However, the BCA method is affected by chelating agents and slightly higher concentrations of reducing agents. It is necessary to ensure that EDTA is less than 10 mM, no EGTA, dithiothreitol (DTT) is less than 1 mM, and beta-mercaptoethanol (beta-Mercaptoethanol) is less than 0.01%. 3. It is recommended to make a standard curve for each measurement. Because the color of the BCA method will continue to deepen with the extension of time, and the color reaction will be accelerated due to the increase of temperature. 4. If the sample diluent or lysate itself has a high background, please try the Protein Quantification Kit (Bradford Assay) (Cat #:KTD3002).
Detection Method	Colorimetric
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis.
Notes	The BCA method is suitable for the detection of protein concentrations in the range of 20-2,000 ug/mL, the minimum detected protein amount is 0.4 ug, and the sample volume to be tested is 1-20 uL.
Kit Components	BCA Reagent A BCA Reagent B BSA Standard BSA Standard Buffer
Limit Of Detection	20 ug/mL

Antigen	BCA

Storage	Store at room temperature, stable for 12 months

Description	Abbkine Protein Quantification Kit (BCA Assay) provides a simple, rapid, detergent-compatible procedure for the colorimetric detection and quantitation of total protein. The method utilizes the reduction of the copper cation (Cu2+) to the cuprous cation (Cu+) by protein in an alkaline medium. The generated Cu+ forms an intensely purple-colored complex with the bicinchoninic acid(BSA) with a very strong absorbance at 562nm. The intensity of the water-soluble complex is proportional to the amount of protein in the sample. The BCA Protein Assay is suitable for measuring protein concentration in the range of 50-1000µg/ml.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13700005	KTD3001	500 T	$69		Add to Cart
BHE13700005	KTD3001	2500T	$169		Add to Cart

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