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Home / Proteins & Peptide

PRMT4 (CARM1), FLAG-tag (HEK293-derived)

SKU : BHP18200426

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BPS Bioscience

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Mfr.No.	51047
Description	Human PRMT4 (CARM1), GenBank Accession No. NM_199141, a.a. 2-end, with N-terminal FLAG-tag, MW=66 kDa, expressed in FreeStyle 293-F cells. host species: human Host species: human Host cell line: FreeStyle 293-F cells
Background	CARM1 (PRMT4) methylates (mono- and asymmetric dimethylation) the arginine residues in several proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability. It is recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at Arg17 (H3R17me), forming mainly asymmetric dimethylarginine (H3R17me2a), resulting in transcription activation via chromatin remodeling. CARM also methylates arginines in several other proteins, including EP300/P300 at Arg-2142, Arg-580, and Arg-604. CARM1 acts as coactivator for PPARG, and seems to be involved in p53/TP53 transcriptional activation.
Immunogen Region	2-end
Tag	N-terminal FLAG-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 80 ng/ul FLAG peptide
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	50 ul reaction mix (1x HMT Assay Buffer 5, BPS catalog # 52191 and 5-1000 ng methyltransferase PRMT4) is added to wells coated with the substrate on a Thermo HBC Neutravidin black plate. Incubate at room temperature for 1 hr. Add antibody against methylated R3 residue of histone H4, incubate 1 hr. Add secondary HRP-labeled antibody and incubate 30 min. Finally, add HRP chemiluminescent substrates and read luminesence. Avoid freeze/thaw cycles.
GenBank	NM_199141
Mw(kda)	66 kDa

Synonyms	PRMT4, CARM1, Coactivator-Associated Arginine Methyltransferase 1
Uniprot	Q86X55

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Li H. et al., J. Biol. Chem. 277 (47), 44623-44630 (2002). 2. Frietze S. et al., Cancer Res. 68 (1), 301-306 (2008).

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200426	51047	20 ug	$495		Add to Cart

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