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Home / Proteins & Peptide

PRMT1, GST-tag (Sf9-derived)

SKU : BHP18200423

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BPS Bioscience

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Mfr.No.	51041
Description	Human PRMT1 (GenBank Accession No. NM_001536), a.a. 2-end, with N-terminal His-GST-tag, MW= 68 kDa, expressed in a Baculovirus infected Sf9 cell expression system. host species: insect Host species: insect Host cell line: Sf9 Specific activity: 6.2 pmol/min/ug
Background	PRMT1 is a protein arginine methyltransferase that functions as a histone methyltransferase specific for histone H4. PRMT1 specifically methylates arginine-3 of histone H4 in vitro and in vivo. Methylation of arg3 by PRMT1 facilitates subsequent acetylation of histone H4 tails by p300. PRMT1 performs methylation of arg31 of STAT1 and this is required for transcription induced by IFN-alpha/IFN-beta. Arginine methylation of STAT1 is an additional posttranslational modification regulating transcription factor function, and alteration of arginine methylation might be responsible for the lack of interferon responsiveness observed in many malignancies. Type I PRMTs ((CARM1, PRMT1, PRMT2, PRMT3, PRMT6, and PRMT8) catalyze asymmetric dimethylarginine (ADMA), while type II PRMTs (e.g. PRMTs 5 and 7) catalyze SDMA. PRMT1 is the primary methyltransferase that deposits the ADMA mark, and it accounts for over 90% of this type of methylation.
Immunogen Region	2-end
Tag	N-terminal His-GST tag
Formulation	40 mM Tris pH 8.0, 300 mM NaCl, 20% glycerol and 1 mM DTT.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Enzyme was incubated with 0.25 uM of biotinylated H4 1-21 peptide with 1 uM AdoMet in HMT buffer 2A in a 10 ul reaction. 5 ul of Anti-Rabbit AlphaLISA acceptor beads (1:250) and 5 ul of Primary Antibody 4 (1:20) were added and incubation continued for 30 min. Streptavidin Donor beads (1:125) were added (10 ul) and mixture was further incubated for 15 min at RT before A-counts were measured. Avoid freeze/thaw cycles.
GenBank	NM_001536
Mw(kda)	68 kDa

Synonyms	PRMT1, HRMT1L2, IR1B4, HCP1
Uniprot	Q99873

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Sun X.J., et al., J Biol Chem. 280, 42, 35261-35271, 2005. 2. Rega S. et al., Mol Cell Neurosci. 18, 1, 68-79, 2001.Application Reference(s): 1.A Sensitive Luminescent Assay for the Histone Methyltransferase NSD1 and Other SAM-Dependent Enzymes (2014) 2.Selective inhibitors of bacterial t-RNA-(N(1)G37) methyltransferase (TrmD) that demonstrate novel ordering of the lid domain (2013)

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200423	51041	20 ug	$363		Add to Cart

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