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Home / Proteins & Peptide

PLPro, His-tag (SARS-CoV-2)

SKU : BHP18201260

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BPS Bioscience

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Mfr.No.	100735-1
Description	SARS-Cov-2 papain-like protease (PLPro), part of a large replicase polyprotein 1ab (E1564-Y1882), GenBank Accession No. QHD43415, with a N-terminal His-tag, expressed in an E. coli expression system. MW=38 kDa.
Immunogen Region	E1654-Y1882
Tag	N-terminal His-tag
Formulation	40 mM Tris-HCl, pH 8, 110 mM NaCl, 2.2mM KCl, 0.04% Tween-20, 3 mM DTT, 20% glycerol and 115 mM Imidazole.
Species	SARS-CoV-2
Application	Useful for studying protein binding and screening small molecular inhibitors
Notes	PLPro (SARS-CoV-2) was diluted with DUB reaction buffer and incubated at room temp for 30 minutes. Plate read using KinaseGlo (Promega, #E7031). Avoid freeze/thaw cycles.
GenBank	QHD43415
Mw(kda)	38 kDa

Synonyms	papain like protease, SARS-CoV, protease, n-CoV 2019 protease, Sars-cov-2, sars cov 2, sarscov2
Uniprot	P0DTD1

Shipping Temperature	-80°C (dry ice)
Storage	At least 6 months at -80°C.

Reference	1. Wu, F., et al., Nature. 2020; 579:265-269. 2. Baez-Santos, Y., et al., Antiviral Res. 2015; 115: 21-38.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18201260	100735-1	100 ug	$385		Add to Cart

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