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Home / Proteins & Peptide

PI3 kinase (p120gamma), His-tag

SKU : BHP18200281

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BPS Bioscience

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Mfr.No.	40625
Immunogen Region	full length
Tag	N-terminal His-tag
Description	Human p120gamma (gamma) (GenBank Accession No. NM_002649), full length with N-terminal His-tag, MW=130 kDa, expressed in a Baculovirus infected Sf9 cell expression system. Host cell line: Sf9 cells Specific activity: 1200 pmol/min/ug.
Formulation	25 mM Tris, pH 8.0, 69 mM NaCl, 1.35 mM KCl, 0.025% Tween-20, 3 mM DTT, and 50% glycerol
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	A 25 ul kinase reaction is conducted in a buffer containing 40 mM Tris-HCl (pH 7.4), 20 mM MgCl₂, 100 uM ATP, 200 uM PI:PS substrate (BPS catalog #40560) and 0-200 ng p120gamma enzyme for 15 minutes at 30°C. ATP reduction is detected using ADP-Glo Kinase Assay Platform (Promega Corporation, Madison, WI). Avoid freeze/thaw cycles.
GenBank	NM_002649
Mw(kda)	130 kDa

Synonyms	PI3CG, 3-kinase catalytic subunit gamma isoform; p120-PI3K; PI3K-gamma, p120gamma
Uniprot	P48736

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Webb, LM., et al., J Immunol. 175(5):2783-7 (2005). 2. Voigt, P., et al., J Biol Chem. 280(6):5121-7(2005).Application Reference:Discovery of a potent and isoform-selective targeted covalent inhibitor of the lipid kinase PI3Kalpha (2013)

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200281	40625	20 ug	$478.50		Add to Cart

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