Human PHF8 also knownas KDM7B (lysine-specific demethylase7B), JHDM1F, and KIAA1111, GenBankAccession No. NM_001184896, full-length(a.a. 2-1060(end)), with N-terminalFLAG-tag, MW=120 kDa,expressed in a Baculovirus-infected Sf9cell expression system.
Host cell line: Sf9 cells
Immunogen Region
2-1060(end)
Tag
N-terminal FLAG-tag
Formulation
40 mM Tris-HCl, pH 8.0, 110mM NaCl, 2.2 mM KCl, 0.04% Tween-20,20% glycerol, 80 ug/ml FLAG peptide
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Avoidfreeze/thaw cycles. Storing diluted enzyme isnot recommended, if necessary, use carrierprotein (BSA 0.1 - 0.5%).
GenBank
NM_001184896
Mw(kda)
120 kDa
Synonyms
KDM7B, JHDM1F, PHD finger protein 8, ZNF422
Uniprot
Q9UPP1
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Liu, W. et al., Nature. 2010; 466(7305): 508-512. 2. Zhu, Z. et al., Cell Res. 2010; 20(7): 794-801.