Human Peroxiredoxin 2, also known as PRDX2, full length (Genbank accession number NM_005809) with N-terminal His-tag, MW=22.7 kDa, expressed in Baculovirus infected Sf9 cell expression system.
Specific activity: >=149 pmol/min/ug
Formulation
25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 1.35 mM KCl, 0.05% Tween-20, 50% glycerol, and 3 mM DTT.
Species
Human
Application
Useful for the study of biochemical function, enzyme kinetics, screening inhibitors, and selectivity profiling.
Notes
3.45 ug of PRDX2 was incubated in 50 mM HEPES (pH 7.0) containing 200 uM NADPH, 3 uM thioredoxin, and 1.5 uM thioredoxin reductase. The reaction mixture was incubated at 30°C for 5 min, followed by the addition of 0.22 mM H2O2. NADPH oxidation was monitored (fluorescence decrease) for the next 10 min.
Avoid freeze/thaw cycles.
GenBank
NM_005809
Mw(kda)
22.7 kDa
Synonyms
PRDX2
Uniprot
P32119
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Peskin,A.V., et al., J. Biol. Chem. 282 (16), 11885-11892 (2007) 2. Low,F.M., et al., Blood 109 (6), 2611-2617 (2007)