Human Peroxiredoxin I (GenBank Accession No. NM_002574), full length, with N-terminal His- tag, MW=23 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Specific activity: >=896 pmol/min/ug
Immunogen Region
full length
Tag
N-terminal His-tag
Formulation
25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, 3 mM DTT.
Species
Human
Application
Useful for the study of biochemical function, enzyme kinetics, screening inhibitors, and selectivity profiling.
Notes
1.88 ug of PRDX1 was incubated in 50mM HEPES (pH 7.0) containing 200 uMNADPH, 3 uM thioredoxin, and 1.5 uMthioredoxin reductase. The reactionmixture was incubated at 30°C for 5min, followed by the addition of 0.22mM H2O2. NADPH oxidation wasmonitored (fluorescence decrease) for thenext 10 min.
Avoid freeze/thaw cycles.
GenBank
NM_002574
Mw(kda)
23 kDa
Synonyms
PRDX1
Uniprot
Q06830
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Maksimov,V. et al. Genetika 42 (2), 274-277 (2006) 2. Jang,H.H et al. FEBS Lett. 580 (1), 351-355 (2006)