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Home / Proteins & Peptide

PDE4B2, GST-tag, His-tag

SKU : BHP18200508

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BPS Bioscience

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Mfr.No.	60042
Description	Human PDE4B2 (GenBank Accession No. NM_001037339), full length, with N-terminal GST-tag and C-terminal His-tag, MW=91.2 kDa, expressed in a Baculovirus infected Sf9 cell expression system. host species: Sf9 cells Host species: Sf9 cells Specific activity: >= 12,300 pmol/min/ug Unit definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.
Immunogen Region	full length
Tag	N-terminal GST-tag, C-terminal His-tag
Formulation	40 mM Tris-HCl, pH 8.0,110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, and 20% glycerol.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Assayed using BPS's PDE4B2 Assay Kit, Cat. # 60343. Assay conditions: 10 mM Tris-HCl,pH 7.4, 10 mM MgCl₂, 0.1 mg/ml BSA,0.05% Tween-20, 200 uM cAMP, 2.5 kU 5'-nucleotidase, andserial dilutions of PDE4B2 at 37°C for 20 min.Quantified by 5'- nucleotidase cleaving the5' -AMP product and releasing thephosphate group which is detected byMalachite Green Reagent. Avoid freeze/thaw cycles.
GenBank	NM_001037339
Mw(kda)	91.2 kDa

Synonyms	PDE4B2, phosphodiesterase 4B2
Uniprot	Q07343

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Arp, J., et al., Mol Cell Biol. 2003 Nov; 23(22):8042-57. 2. Wang, P., et al., Mol Pharmacol. 1999 Jul; 56(1):170-4.Application Reference(s): Evaluation of the therapeutic index of a novel phosphodiesterase 4B-selective inhibitor over phosphodiesterase 4D in mice (2013)

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200508	60042	5 ug	$533.50		Add to Cart

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