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Home / Proteins & Peptide

PDE4B1, GST-tag

SKU : BHP18200507

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BPS Bioscience

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Mfr.No.	60041
Description	Human PDE4B1 (GenBank Accession No. NM_001037341), full length with N-terminal GST-tag, MW= 109 kDa, expressed in a Baculovirus infected Sf9 cell expression system. host species: Sf9 cells Host species: Sf9 cells Specific activity: >= 8532 pmol/min/ug Unit definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.
Immunogen Region	full length
Tag	N-terminal GST-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	10 mM Tris-HCl,pH 7.4, 10 mM MgCl₂, 1 mM MnCl₂, 200 uM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of PDE4B1 at 37°C for 20 min.Quantified by 5'- nucleotidase cleaving the5' -AMP product and releasing thephosphate group which is detected byMalachite Green Reagent. Avoid freeze/thaw cycles.
GenBank	NM_001037341
Mw(kda)	109 kDa

Synonyms	PDE4B1, phosphodiesterase 4B1
Uniprot	Q07343

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Shah,V.O., et al., Am. J. Kidney Dis. 48 (4), 616-628 (2006) 2. Smith,P.G., et al., Blood 105 (1), 308-316 (2005).

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200507	60041	10 ug	$517		Add to Cart

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