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Home / Proteins & Peptide

PDE3A, (669-end), His-GST-tags

SKU : BHP18200502

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BPS Bioscience

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Mfr.No.	60032
Immunogen Region	669-1141(end)
Tag	N-terminal His-GST-tags
Description	Human PDE3A (GenBank Accession No. NM_000921), a.a. 669-1141(end) with N-terminal His-GST-tag, MW = 82 kDa, expressed in a Baculovirus infected Sf9 cell expression system. host species: Sf9 cells Host species: Sf9 cells Specific activity: >= 4000 pmol/min/ug Unit definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.
Formulation	40 mM Tris-HCl, pH 8.0,110 mM NaCl, 2.2 mM KCl, 16 mMglutathione, 20% glycerol and 3 mM DTT.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Assayed using BPS's PDE3A Assay Kit, Cat. # 60330. Assay conditions: 10 mM Tris-HCl,pH 7.4, 10 mM MgCl₂, 0.1 mg/ml BSA,0.05% Tween-20, 200 uM cAMP, 2.5 kU 5'-nucleotidase, andserial dilutions of PDE3A at 37°C for 20 min.Quantified by 5'- nucleotidase cleaving the5' -AMP product and releasing thephosphate group which is detected byMalachite Green Reagent. Avoid freeze/thaw cycles.
GenBank	NM_000921
Mw(kda)	82 kDa

Synonyms	PDE3A, phosphodiesterase 3A
Uniprot	Q14432

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Hung, S.H., et al. J. Biol. Chem. 281 (39), 29236-29244 (2006) 2. Hambleton, R., et al. J. Biol. Chem. 280 (47), 39168-39174 (2005)Application Reference(s): 1.Characterization of the binding properties of T-773 as a PET radioligand for phosphodiesterase 10A (2015) 2.Analysis of substrate specificity and kinetics of cyclic nucleotide phosphodiesterases with N'-methylanthraniloyl-substituted purine and pyrimidine 3',5'-cyclic nucleotides by fluorescence spectrometry (2013)

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200502	60032	5 ug	$500.50		Add to Cart

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