Recombinant human PARP4 (poly(ADP-ribose) polymerase family member 4), encompassing amino acids 2-700. This construct contains an N-terminal His-tag (6xHis) followed by a GST-tag and a Thrombin Cleavage sight to facilitate removal of the GST-tag. This recombinant protein was affinity purified. Constructs: PARP4 (His-GST-Th-2-700)
host species: Sf9
Host species: Sf9
Immunogen Region
2-700
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, and 3 mM DTT
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Notes
Assay was done according to PARP12 Chemiluminescent Assay Kit (Biohippo #78504) with various amounts of PARP4.
Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.