Human PARP12 (GenBank Accession No. NM_022750), a.a. 500-701(end) with N-terminal His-GST-tag, MW = 51 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Unit definition: One unit of PARP incorporates 100 pmoles of poly(ADP) in 1 minute from NAD into the acid-insoluble form.
Immunogen Region
500-701(end)
Tag
N-terminal His-GST tag
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 8 mM glutathione, 0.04% Tween-20, and 20% glycerol.
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Ribosylationreaction is conducted in wells coated with amixture of histones at room temperature for 1hour. Assay buffer: 50 mM Tris-HCl (pH 8.0),5 mM MgCl2 and 20 uM ZnCl2 .