Human PADI-4, also known as Peptidyl arginine deiminase, type IV, (GenBank Accession No. NM_012387), 1-663(end), with C-terminal FLAG-tag, MW= 75 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Host cell line: Sf9 cells
Specific activity: >=80 pmole/min/ug
Immunogen Region
1-663(end)
Tag
C-terminal FLAG-tag
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 80 ug/ml FLAG peptide
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Enzyme reaction was performed in a buffer (50 mM Tris, pH 7.4, 50mM NaCl, 10 mM CaCl2, and 2 mMDTT) containing PADI4 and 10 mMBAEE as a substrate for 25 min at 37°C.Amount of ammonia produced wasmeasured and used for specific activitycalculation.
Avoid freeze/thaw cycles.
GenBank
NM_012387
Mw(kda)
75 kDa
Synonyms
PADI-4, Peptidyl arginine deiminase, type IV, Pad
Uniprot
Q9UM07
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Kanno T, et al. (2000). J. Invest. Dermatol. 115 (5): 813-23. 2. Nachat R, et al. (2005). J. Invest. Dermatol. 124 (2): 384-93. 3. Wang Y, et al. (2004). Science 306 (5694): 279-83.