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Home / Proteins & Peptide

N-Formyl-Met-Leu-Phe

SKU : BHP21300053

A Potent Agonist of FPR1 G-Protein Coupled Receptor

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Mfr.No.	GPF-100, GPF-100_10 mg, GPF-100_100 mg, GPF-100_5 mg, GPF-100_50 mg, GPF-100_500 mg
Description	A Potent Agonist of FPR1 G-Protein Coupled Receptor
Reconstitution	Centrifuge the vial (10,000 x g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. Soluble in DMSO. Prepare a concentrated stock solution by dissolving the lyophilized peptide in DMSO first (e.g., at a concentration between 100-1000x of the final working concentration). Once the peptide is completely dissolved in DMSO, slowly dilute the solution into the working buffer (or water) to the desired final working concentration. Centrifuge all product preparations before use. Avoid multiple freeze-thaw cycles to maintain biological activity. It is recommended to keep the DMSO concentration as low as possible. For cell assays, a final concentration of 0.1%0.5% DMSO (v/v) is considered safe. For other experiments, a 5% DMSO (v/v) concentration is recommended.
Molecular Formula	C21H31N3O5S
Background	Chemotactic factors from both Gram-positive and Gram-negative bacteria are short peptides with N-formyl methionine at the N-terminus (extensively reviewed in reference 1). These peptides are released from bacteria during infection and activate formyl peptide receptors (FPR), members of the G-protein coupled receptor (GPCR) superfamily. In humans, the FPR family consists mainly of three receptors, FPR1, FPR2/ALX (formerly FPRL1), and FPR3 (formerly FPRL2) which all couple to the Gi subtype of G-proteins and ultimately lead to the activation of phospholipase C and intracellular Ca2+ increase1,2.N-Formyl-Met-Leu-Phe is a selective and potent agonist of the Formyl peptide receptor (FPR1)3.In human polymorphonuclear leukocytes N-formyl-met-leu-phe activates p38 by a process involving phosphatidylinositol 3-kinase, protein kinase C, and calcium4. N-Formyl-Met-Leu-Phe increased in a dose-dependent manner (0.1 nM - 1 uM) the adherence of neutrophils to vascular endothelial cells which is the initial event in the migration of neutrophils through blood vessel walls to tissue sites of inflammation5.
Biological Activity	N-Formyl-Met-Leu-Phe is a selective and potent agonist of the formyl peptide receptor (FPR1)1.
Formulation	Lyophilized Powder.
Molecular Weight	437.6 Da
Solubility	Centrifuge the vial before adding solvent (10,000 x g for 5 minutes). The lyophilizate may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. Soluble in DMSO. Prepare a concentrated stock solution by dissolving the lyophilized peptide in DMSO first (e.g., at a concentration between 100-1000x of the final working concentration). Once the peptide is completely dissolved in DMSO, slowly dilute the solution into the working buffer (or water) to the desired final working concentration. Centrifuge all product preparations before use. It is recommended to keep the DMSO concentration as low as possible. For cell assays, a final concentration of 0.1%0.5% DMSO (v/v) is considered safe. For other experiments, a 5% DMSO (v/v) concentration is recommended.
Source	Synthetic peptide
Concentration	1 nM - 10 uM
Form	Lyophilized
Notes	Bioassay Tested: yes Steril endotoxin free: no Is Toxin: No
Cas Number	59880-97-6
UNSPSC Code	12352202
Lead Time	1-2 Business Days
Modifications	Met1 - N-formyl-Met.

Synonyms	FMLP, N-formyl-L-methionyl-L-leucyl-L-phenylalanine, N-formyl-MLF
Gene Id	FPR1
Sequence	MLF-OH
Target	FPR1 receptor

Country Of Origin	Israel/IL
Purity	>=98% (HPLC)
Storage	Storage After Reconstitution: Store the reconstituted solution for the shortest time possible at -20°C. We do not recommend storing the product in working solution for longer than one day. Avoid multiple freeze-thaw cycles. Storage Before Reconstitution: The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture. Storage of solutions: Store the reconstituted solution at -20°C for the shortest time possible. Avoid multiple freeze-thaw cycles. We do not recommend storing the product in working solutions for longer than a day.

Applications	Calcium imaging assay

Reference	Ye, R.D. et al. (2009) Pharmacol. Rev. 61, 119. Le, Y. et al. (2002) Trends Immunol. 23, 541. Showell, H.J. et al. (1976) J. Exp. Med 143, 1154. Krump, E. et al. (1997) J. Biol. Chem. 272, 937. Tonnesen, M.G. et al. (1984) J. Clin. Invest. 74, 1581.

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHP21300053-1	GPF-100_10 mg	10 mg	$55		Add to Cart
BHP21300053-2	GPF-100_100 mg	100 mg	$261		Add to Cart
BHP21300053-3	GPF-100_5 mg	5 mg	$37		Add to Cart
BHP21300053-4	GPF-100_50 mg	50 mg	$129		Add to Cart
BHP21300053-5	GPF-100_500 mg	100 mg	$707		Add to Cart

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