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Mycobacterium bovis BCG Recombinant HSP65 Protein

SKU : BHP11900028

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StressMarq Biosciences Inc.

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Mfr.No.	SPR-116, SPR-116A, SPR-116B, SPR-116C
Description	Mycobacterium bovis BCG Recombinant HSP65 Protein
Alternative Names	60kDa chaperonin 2 Protein, Antigen A Protein, Cell wall protein A Protein, groEL Protein, GroEL2 Protein, GroL2 Protein, M. Tuberculosis cell wall protein A Protein, M. Tuberculosis HSP65 Protein, Protein Cpm60 2 Protein
Cellular Localization	Cytoplasm
Certificate Of Analysis	This product has been certified >90% pure using SDS-PAGE analysis.
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For research use only.
Regulatory	RUO
Scientific Background	HSP65 isolated from Mycobacterium bovis BCG, is a member of the HSP60 family of heat shock proteins (2, 3). HSP60s are mitochondrial chaperonins that are typically held responsible for the transportation and refolding of proteins from the cytoplasm into the mitochondrial matrix. In addition to its role as a heat shock protein, HSP60 functions as a chaperonin to assist in folding linear amino acid chains into their respective three-dimensional structure. HSP60s are a ubiquitous class of HSPs that specifically promote the folding and assembly of cellular polypeptides in an ATP-dependent manner (1). Specifically, sequence comparison of HSP65 from different mycobacterium strains showed that the protein sequence of M. bovis BCG is identical to that of M. tuberculosis, and very similar to that of M. leprae, the pathogens that cause tuberculosis and tuberculoid leprosy, respectively (2,4). Mycobacterium bovis BCG HSP65 was identified as the immunodominant antigen during mycobacterial diseases and vaccination. It is also believed to be the antigen that induces autoimmune disease, such as adjuvant arthritis in rats (5, 6).
Species	Bacteria
Specificity	~65 kDa
Accession Number	M17705.1
Concentration	Lot/batch specific. See included datasheet.
Purification	Multi-Step Purified
Expression System	E. coli
Amino Acid Sequence	MAKTIAYDEEARRGLERGLNALADAVKVTLGPKGRNVVLEKKWG APTITNDGVSIAKEIELEDPYEKIGAELVKEVAKKTDDVAGDGTTTATVLAQALVREG LRNVAAGANPLGLKRGIEKAVEKVTETLLKGAKEVETKEQIAATAAISAGDQSIGDLI AEAMDKVGNEGVITVEESNTFGLQLELTEGMRFDKGYISGYFVTDPERQEAVLEDPYI LLVSSKVSTVKDLLPLLEKVIGAGKPLLIIAEDVEGEALSTLVVNKIRGTFKSVAVKA PGFGDRRKAMLQDMAILTGGQVISEEVGLTLENADLSLLGKARKVVVTKDETTIVEGA GDTDAIAGRVAQIRQEIENSDSDYDREKLQERLAKLAGGVAVIKAGAATEVELKERKH RIEDAVRNAKAAVEEGIVAGGGVTLLQAAPTLDELKLEGDEATGANIVKVALEAPLKQ IAFNSGLEPGVVAEKVRNLPAGHGLNAQTGVYEDLLAAGVADPVKVTRSALQNAASIA GLFLTTEAVVADKPEKEKASVPGGGDMGGMDF
Cite This Product	Mycobacterium bovis BCG Recombinant HSP65 Protein (StressMarq Biosciences, Canada, Cat # SPR-116A)
Mol Weight	~65 kDa
Nature	Recombinant
Protein Length	Full Length

Gross Weight (kg)	1.4
Launch Date	2008/05/01
Net Weight (g)	0.05
Number Of Pubmed Citations	6
Swissprot	P0A521
Target Details	HSP65
Research Area	Cancer, Heat Shock
Target	HSP65

Adr Code	Non-hazardous
Country Of Origin	Canada
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Published Application	Protein Binding Assay ; Functional Assay ; Functional Assay ; ELISA ; Functional Assay ; Weste Blot Control
Published Species Reactivity	Mouse ; Human ; Mouse ; M. bovis ; Mouse ;
Pubmed Id	28115505 ; 27742830 ; 17920518 ; 26925118 ; 25463133 ; 24066722
Shipping	Blue Ice or 4ºC
Stock Product	Y
Tarriff Code	3822.19.0030
Un Code For Transport	Non-hazardous
Unspsc Code	12352202
Shipping Temperature	Blue Ice or 4°C
Buffer	20mM Tris, 150mM NaCl, 10% glycerol
Description	Accession Number: AAQ64501.1 Specificity: ~65 kDa Certificate of Analysis: This product has been certified >90% pure using SDS-PAGE analysis. Country of Origin: Canada Gross Weight (kg): 1.4 Net Weight (g): 0.05 Stock Product: Y
Purity	>90%
Storage Buffer	20mM Tris/HCl, pH 7.5, 0.45M NaCl, 10% glycerol, 5mM bMe
Storage Temperature	-20°C

Immunogen Species	Bacteria (Eubacteria)
Applications	Functional assay, WB, ELISA, SDS-PAGE

Reference	1. Koll H., et al. (1992) Cell. 68: 1163-1175. 2. Thole J.E.R., et al. (1985) Infect. Immuno. 50: 800-806. 3. Thole J.E.R., et al., (1987) Infect. Immuno. 55: 1466-1475. 4. Shinnick T.M. Sweetser D., Thole J., van Embden J. and Young R.A. (1987) Infect. Immuno. 55: 1932-1935. 5. Van Eden W., et al. (1988) Nature 331: 171-178. 6. Cobelens P.M., et al. (2002) Rheumatology 41: 775-779.

Product Manual	Datasheet
SDS

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHP11900028-1	SPR-116A	50 ug	$241		Add to Cart
BHP11900028-2	SPR-116B	100 ug	$341		Add to Cart
BHP11900028-3	SPR-116C	100 ug x2	$647		Add to Cart

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