Recombinant mouse VEGF (GenBank Accession No. NM_009505) is a 39 kDa disulfide-linked homodimeric protein (a.a. 27-190), consisting of two 164 amino acid polypeptide.
host species: E. coli
Host species: E. coli
Reconstitution
Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.
Immunogen Region
27-190
Tag
none
Formulation
Lyophilized from 0.2 um filtered 20 mM PBS solution, pH 7.0
Species
Mouse
Application
Useful for cell culture and for the study of signaling and developmental pathways. Also useful for receptor binding studies, screening inhibitors, and selectivity profiling.
Endotoxin Level
< 1EU per ug of VEGF as determined by the LAL method.
Notes
Avoid freeze/thaw cycles.
Biological Activity Comment
ED50 is less than 2 ng/ml as determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC).
GenBank
NM_009505
Mw(kda)
39 kDa
Synonyms
Vascular endothelial growth factor 165, VEGF
Uniprot
Q00731
Shipping Temperature
-20°C or -80°C
Format
lyophilized protein
Purity
>=98% by SDS-PAGE and HPLC analysis.
Storage
>=2 years (lyophilized) at -20°C. Reconstituted solutions can be stored in undiluted aliquots at 4°C forone week or at -20°C for up to six months.