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Home / Proteins & Peptide

Mouse Cot (MAP3K8), His-GST-tags

SKU : BHP18200298

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BPS Bioscience

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Mfr.No.	41050
Description	Mouse Cot kinase (MAP3K8), GenBank Accession No. NM_007746, a.a. 30-397, with N-terminal His-GST-tag, expressed in a Baculovirus infected Sf9 cell expression system. Theoretical MW is 70 kDa. Host cell line: Sf9 cells Specific activity: 8.5 pmole/min/mg
Background	COT is an oncogene that can activate both the MAP kinase and JNK kinase pathways. COT activates IkappaB kinases and induces the nuclear production of NF-kappaB. C-terminal catalytic domain of KSR2 associates with COT and KSR2 can negatively regulates the kinase activity of COT in vitro. Co-transfection of KSR2 with COT in cells lead to reduced COT-mediated ERK activation and COT-induced IL8 production in a dose-dependent manner. Cot is one of the MAP kinase kinase kinases that regulates the ERK1/ERK2 pathway in response to IL-1. Blockage of expression of Cot results in failure of IL-1 to induce an increase in IL-8 and MIP-1betamRNA levels.
Immunogen Region	30-397
Tag	N-terminal His-GST-tags
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.4 mM KCl, 5 mM glutathione, 134 mM imidazole, 3 mM DTT and 20% glycerol.
Species	Mouse
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Reaction was performed in a kinase buffer (40 mM Tris, 10 mM MgCl₂, 0.1 mg/ml BSA) containing 5 ug inactive MEK1 (BPS #40075), 20 uM ATP, and mCOT for 60 minutes at 30°C. The amount of ATP transferred was measured by Kinase-Glo plus (Promega). Avoid freeze/thaw cycles.
GenBank	NM_007746
Mw(kda)	70 kDa (calculated)

Synonyms	COT, TPL2, MEKK8, mitogen-activated protein kinase kinase kinase 8, Cancer Osaka Thyroid oncogene, Ewing Sarcoma Transformant, EST, Tumor Progression Locus 2, MAP3K8
Uniprot	Q07174

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Jeong, J.H. et al., PLoS One. 2011 Jan 18, 6(1):e16205. 2. Soria-Castro, I. et al. J Biol Chem. 2010 Oct 29,285(44):33805-15.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200298	41050	10 ug	$390.50		Add to Cart

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