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Home / Proteins & Peptide

MMP3(K45E), His-tag

SKU : BHP18200012

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Mfr.No.	11346
Description	Human matrix metallopeptidase-3 (MMP3), also known as Stromelysin-1 (STMY1), GenBank Accession No. NM_002422, a.a. 18-477(end) with a Lys to Glu mutation at a.a. 45 and a C-terminal His-tag, expressed in a HEK293 cell expression system. MW=54 kDa. host species: human Host species: human Host cell line: HEK293
Immunogen Region	18-477(end)
Tag	C-terminal His-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200 mM Imidazole, and 20% glycerol.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Assay buffer: 25 mM Tris pH 9.0, 25 uM ZnCl2, 0.05% Tween-20 Enzyme was diluted to 100 ng/ul in a solution containing 1 mM AMPA (amino-phenyl mercuric acetate) in assay buffer activated for 1 hour at 37°C. Then, 25 ul of 2X 2-fold serial dilution enzyme titration in assay buffer was mixed with 25 ul of 20 uM substrate solution (Mca-RPKPVE-Nval-WRK(Dnp)-NH2 Fluorogenic MMP Substrate, R&D Systems, ES002, 10 mg/ml = 6 mM stock in DMSO, 10 uM final assay concentration) in a Corning 384-well HV black microplate. Fluorescence was kinetically recorded at 320 nm excitation/405 nm emission for 30 minutes. Avoid freeze/thaw cycles.
GenBank	NM_002422
Mw(kda)	54 kDa

Synonyms	Stromelysin-1, Stromelysin, STMY
Uniprot	P08254

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Sternlicht, M.D., et al., Cell, 1999; 98(2):137-146. 2. Buttice, G., et al., Oncogene. 1996; 13(11):2297-2306.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200012	11346	20 ug	$467.50		Add to Cart

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