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Home / Proteins & Peptide

MIP-1beta /CCL4, Human

SKU : BHP18500405

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iXCells Biotechnologies

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Mfr.No.	IX03264, IX03264-1, IX03264-25, IX03264-5
Description	Macrophage inflammatory protein 1 beta (MIP-1beta), also known as Chemokine (C-C motif) ligand 4(CCL4), is a small cytokine belonging to the CC chemokine family. It is a chemoattractant for natural killer cells, monocytes and a variety of other immune cells. MIP-1betais a major HIV-suppressive factor produced by CD8+ T cells. Perforin-low memory CD8+ T cells are the most common T-cells that normally synthesize MIP-1-beta in humans. MIP-1betahas been shown to interact with CCL3. It can signal through the CCR5 receptor.Recombinant MIP-1 beta/CCL4 produced in E. coli is a single non-glycosylated polypeptide chain containing 69 amino acids. A fully biologically active molecule, rhMIP-1 beta/CCL4 has a molecular mass of 7.6 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques.
Reconstitution	Reconstituted in ddH2O or PBS at 100 ug/ml.
Formulation	Lyophilized after extensive dialysis against PBS.
Molecular Weight	7.6 kDa, observed by reducing SDS-PAGE.
Source	E. coli
Host	Human
Accession Number	P13236
Endotoxin Level	0.2 EU/ug, determined by LAL method.
Notes	For research use only
Bioactivity	The EC50 value of human MIP-1 beta /CCL4 on Ca2+ mobilization assay in CHO-K1/ Galpha15/hCCR5 cells (human Galpha15 and human CCR5 stably expressed in CHO-K1 cells) is less than 100 ng/ml.

Synonyms	Macrophage Inflammatory Protein-1?, CCL4, ACT-2
Sequence	APMGSDPPTA CCFSYTARKL PRNFVVDYYE TSSLCSQPAVVFQTKRSKQV CADPSESWVQ EYVYDLELN

Purity	95% as analyzed by SDS-PAGE and HPLC.
Storage	Lyophilized recombinant human MIP-1 beta /CCL4 remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, human MIP-1 beta /CCL4 should be stable up to 1 week at 4°C or up to 2 months at -20°C.

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHP18500405-1	IX03264-1	1mg	$1686		Add to Cart
BHP18500405-2	IX03264-25	25ug	$124		Add to Cart
BHP18500405-3	IX03264-5	5ug	$70		Add to Cart

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