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Home / Proteins & Peptide

MCP-1/CCL2, Human

SKU : BHP18500433

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Mfr.No.	IX03292, IX03292-1, IX03292-25, IX03292-5
Description	Chemokine (C-C motif) ligand 2 (CCL2) is also referred to as monocyte chemotactic protein 1 (MCP1) and small inducible cytokine A2. CCL2 is a small cytokine that belongs to the CC chemokine family. CCL2 recruits monocytes, memory T cells, and dendritic cells to the sites of inflammation produced by either tissue injury or infection. Human CCL2 cDNA encodes a 99 amino acid (aa) precursor protein with a 23 aa signal peptide and a 76 aa mature protein. Human CCL2 shares 78-79% aa identity with canine, porcine and equine CCL2, while mouse and rat express a form of CCL2 that is extended by 49 aa and shares only ~56% aa identity within the common region.Recombinant Human MCP-1/CCL2 produced in E.coli is a single non-glycosylated polypeptide chain containing 76 amino acids. A fully biologically active molecule, rhMCP-1/CCL2 has a molecular mass of 8.7 kD analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques.
Reconstitution	Reconstituted in ddH2O or PBS at 100 ug/ml.
Formulation	Lyophilized after extensive dialysis against PBS.
Molecular Weight	8.7 kDa, observed by reducing SDS-PAGE.
Source	E. coli
Accession Number	P13500
Endotoxin Level	0.2 EU/ug, determined by LAL method.
Notes	For research use only
Bioactivity	The EC50 value of human MCP-1/CCL2on Ca2+ mobilization assay in CHO-K1/Galpha15/hCCR2 cells (human Galpha15 and human CCR2 stably expressed in CHO-K1 cells) is less than 1ug /ml.

Synonyms	Monocyte Chemotactic Protein-1, CCL2, MCAF, JE (murine)
Sequence	Gln24-Thr99 (Accession #:P13500)

Purity	95% as analyzed by SDS-PAGE.
Storage	Lyophilized recombinant Human MCP-1/CCL2 remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, human MCP-1/CCL2 should be stable up to 1 week at 4°C or up to 3 months at -20°C.

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHP18500433-1	IX03292-1	1mg	$1686		Add to Cart
BHP18500433-2	IX03292-25	25ug	$124		Add to Cart
BHP18500433-3	IX03292-5	5ug	$50		Add to Cart

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