Human linker for activation of T cells family member 1 (LAT), also known as 36 kDa phospho-tyrosine adapter protein, pp36. GenBank Accession No. NM_001014989* variant 4, a.a. 64-269(end), with N-terminal His-tag, expressed in an E. coli expression system. MW = 24.4 kDa. This protein runs at a higher MW by SDSPAGE due to glycosylation.
Immunogen Region
64-269(end)
Tag
N-terminal His-tag
Formulation
40 mM Tris pH 8.0, 110 mMNaCl, 2.2 mM KCl, 0.04% Tween-20, 20%glycerol and 3 mM DTT.
Species
Human
Application
Useful as a kinase substrate and for screening small molecules and antibodies.
Notes
This corresponds to a.a. 28-233 of variant 2, NM_001014989. The aggregation state of this product has been confirmed to be<10% Avoid freeze/thaw cycles. Protein may be diluted to>=100 ug/ml in PBS + glycerol and stored at -80°C.
GenBank
NM_001014989*
Mw(kda)
24.4 kDa
Synonyms
linker for activation of T cells family member 1, phospho-tyrosine adapter protein, pp36
Uniprot
O43561
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Zhang, W. et al., Cell. 1998; 92(1): 83- 92. 2. Balagopalan, L. et al., J. Immunol. 2013; 190(8): 3849-53.