Recombinant human RAS (GTPase), isoform b, encompassing amino acids 2-185. This construct contains an N-terminal His-tag (6xHis). The protein also contains mutation of interest G12V. The protein (Biohippo #100480<) was affinity purified and loaded with GDP. Unbound GDP was removed by spin column. Constructs: KRAS (G12V) (His-2-185) - (GDP)
Immunogen Region
2-185
Tag
N-terminal His-tag
Formulation
20 mM HEPES, pH 7.4, 150 mM NaCl, 1 mM DTT
Mutation
G12V
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Assay was performed according to KRAS(G12C) Nucleotide Exchange Assay Kit (Biohippo #79859) with various amounts of KRAS (G12V), His-Tag, GDP-Loaded.