JavaScript is currently not supported/disabled by this browser. Please enable JavaScript for full functionality.

Call Us 1 - 866 - 986 - 9598
orders@biohippo.com

Sign Up

Login
- My Order
- Return Requests
- Track my Order

Home / Proteins & Peptide

KRAS (G12D), Isoform A, His-Tag, GppNHp-Loaded Recombinant

SKU : BHP18201083

Contact us to order

Tel

+1 866.986.9598

Email

orders@biohippo.com

Get a Quote

Request More Infos

BPS Bioscience

view supplier details

Order Now

Credit card payments now incur a 3% fee.

Contact us to order

Tel

+1 866.986.9598

Email

orders@biohippo.com

Get a Quote

Ask Merchant

Request More Infos

Sample Request

Mfr.No.	101481
Description	Recombinant human KRAS (KRAS proto-oncogene GTPase), isoform a, encompassing amino acids 2-186(end). This construct contains an N-terminal His-tag (6xHis) followed by an optimal TEV protease target sequence that allows cleavage of the His-tag from the protein (TEV: tobacco etch virus cysteine protease, Biohippo #50308). The protein also contains mutation of interest G12D. The protein was affinity purified and loaded with GppNHp, a non-hydrolyzable GTP analog. Unbound GppNHp was removed by spin column. Ready for use in KRAS-RAF binding studies or inhibitor assays. Constructs: KRAS (G12D) (His-TEV-2-186(end))-(GppNHp) host species: E. coli Host species: E. coli
Immunogen Region	2-186(end)
Tag	N-terminal His-tag
Formulation	20 mM HEPES, pH 7.4, 150 mM NaCl, and 1 mM DTT
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Notes	Assay was performed according to KRAS(G12C) Coupled Nucleotide Exchange Assay Kit (Biohippo #78004) without SOS1/GTP mixture. KRAS (G12D), Isoform A, His-Tag, GDP-Loaded Recombinant (Biohippo #101312) and GppNHP loaded KRAS (G12D) (Biohippo #101481) were titrated at various concentrations. Avoid freeze/thaw cycles.
GenBank	NM_033360
Mw(kda)	23 kDa

Synonyms	GTPase KRas, K-Ras 2, Ki-Ras, c-K-ras, c-Ki-ras, GTPase KRas, N-terminally processed, KRAS2, RASK2
Uniprot	P01116

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Purity	>=90%
Storage	At least 6 months at -80°C.

Product Manual	Datasheet

Have you used this product?

Rate it now..!!

Login to Review

SKU	Supplier No.	Size	Your price	Quantity
BHP18201083	101481	4 x 60 ug	$1094.50		Add to Cart

ebiohippo.com provides biomedical researchers with a one-stop informatic shopping experience by transforming biotechnology products’ data into a ready-to-analyze state. We collect and unify suppliers’ data on a single platform to reduce the time of searching, uncertainty, and cost of scientific experiments.

© 2024 Biohippo. All rights reserved.