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Home / Proteins & Peptide

JMJD3 (KDM6B), FLAG-tag

SKU : BHP18200339

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Mfr.No.	50115
Description	Human JMJD3 (KDM6B) histone demethylase (a.a. 1043-end), Genbank Accession No. NM_001080424, with C-terminal FLAG-tag, MW=71 kDa, expressed in Sf9 cells via a baculovirus expression system. Host cell line: Sf9 cells Specific activity: 0.022 pmole/min/ug
Immunogen Region	1043-end
Tag	C-terminal FLAG-tag
Formulation	45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 90 ug/ml FLAG peptide, 10% glycerol, and 3 mM DTT.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	10 ul reaction mix containing assay buffer with 20 mM HEPES (pH 7.4), 150 mM NaCl 500 uM alpha-ketoglutarate, 25 uM iron, 2 mM ascorbic acid, 0.01% Tween-20, 0.5 uM biotinylated peptide substrate, and JMJD3 (100 - 500 ng) added to the wells. Add antibody against demethylated K27 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by Alpha Screening detection. Avoid freeze/thaw cycles.
GenBank	NM_001080424
Mw(kda)	71 kDa

Synonyms	JMJD3, KDM6B
Uniprot	O15054

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Y. Xiang, et al., Cell Research (2007) 17:850-8572. 2. S. Hong, et al. Proc. Natl. Acad. Sci. USA (2007) 104(47):18439-44. 3. Swigut T, Wysocka J. Cell (2007) 131(1):29-3.Application References: 1.A small molecule modulates Jumonji histone demethylase activity and selectively inhibits cancer growth (2013) 2.Identification of small molecule inhibitors of Jumonji AT-rich interactive domain 1B (JARID1B) histone demethylase by a sensitive high throughput screen (2013)

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200339	50115	20 ug	$522.50		Add to Cart

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