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Home / Proteins & Peptide

JMJD2A(KDM4A) (1-350), His-tag

SKU : BHP18200347

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BPS Bioscience

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Mfr.No.	50123
Immunogen Region	1-350
Tag	N-terminal His-tag
Description	Human JMJD2A, also known as JHDM3A and KDM4A (GenBank Accession No. NM_014663) (a.a. 1-350) with N-terminal His-tag, MW=42 kDa, expressed in an E. coli expression system. Specific activity: 0.084 pmole/min/ug
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 250 mM imidazole, and 20% glycerol
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	10 ul reaction mix containing assay buffer with 20 mM HEPES (pH 7.4), 150 mM NaCl, 500 uM alpha-ketoglutarate, 25 uM iron, 2 mM ascorbic acid, 0.01% Tween-20, 0.5 uM biotinylated peptide substrate, and JMJD2A (100 - 400 ng) added to the wells. Add antibody against demethylated K9 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by Alpha Screening detection. Avoid freeze/thaw cycles.
GenBank	NM_014663
Mw(kda)	42 kDa

Synonyms	JMJD2A, JHDM3A, KDM4A
Uniprot	O75164

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Zhang, D., et al. (2005). Mol. Cell Biol. 25(15): 6404-6414. 2. Gray SG, et al. (2005). J. Biol. Chem. 280 (31): 28507-18.Application Reference(s): A scintillation proximity assay for histone demethylases (2014)

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200347	50123	100 ug	$511.50		Add to Cart

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