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Home / Proteins & Peptide

JMJD1A (KDM3A), FLAG-tag

SKU : BHP18200350

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BPS Bioscience

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Mfr.No.	50130
Immunogen Region	2-1322 (end)
Tag	N-terminal FLAG-tag
Description	Human JMJD1A (also known as KDM3A or JHDM2A), GenBank Accession No. NM_018433, a.a. 2-1322 (end), with N-terminal FLAG-tag, MW=148.2 kDa, expressed in a Baculovirus infected Sf9 cell expression system. Host cell line: Sf9 cells Specific activity: 0.3 pmole/min/ug
Formulation	45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 90 ng/ul FLAG peptide, and 10% glycerol.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	10 ul reaction mix containing assay buffer with 20 mM HEPES (pH 7.4), 150 mM NaCl, 500 uM alpha-ketoglutarate, 25 uM iron, 2 mM ascorbic acid, 0.01% Tween-20, 0.5 uM biotinylated peptide substrate, and JMJD1A (20 - 100 ng) added to the wells. Add antibody against mono-methylated K9 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by Alpha Screening detection. Avoid freeze/thaw cycles.
GenBank	NM_018433
Mw(kda)	148.2 kDa

Synonyms	JMJD1A, KDM3A, JHDM2A
Uniprot	Q9Y4C1

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Du ZM. et al., PLoS One.; 6(4) : e19137 (2011). 2. Guo X. et al., Neoplasma.; 52(2): 153-157 (2011).Application Reference(s): A scintillation proximity assay for histone demethylases (2014)

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200350	50130	20 ug	$511.50		Add to Cart

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