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Home / Proteins & Peptide

JHDM1D (KDM7A), FLAG-tag

SKU : BHP18200398

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BPS Bioscience

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Mfr.No.	50419
Description	Human JHDM1D, alsoknown as Lysine-specific demethylase 7A(KDM7A) and KIAA1718, GenBankAccession No. NM_030647 a.a. 2-488with an N-terminal FLAG-tag, MW=56kDa, expressed in a baculovirus-infectedSf9 cell expression system. Host cell line: Sf9 cells Specific activity: 0.17 pmole/min/ug
Immunogen Region	2-488
Tag	N-terminal FLAG-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, 80 ug/ml FLAG peptide
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Reaction wasperformed with 20 mM HEPES (pH 7.4),150 mM NaCl, 500 uM -ketoglutarate, 25uM iron, 2 mM ascorbic acid, 0.01%Tween-20, 0.5 uM biotinylated peptidesubstrate, and JHDM1D added to thewells. Add antibody against methylatedH3K9. Incubate for 30 min followed byAlphascreen detection. Avoidfreeze/thaw cycles. Storing dilutedenzyme is not recommended, ifnecessary, use carrier protein (BSA 0.1- 0.5%).
GenBank	NM_030647
Mw(kda)	56 kDa

Synonyms	Lysine-specific demethylase 7A, KDM7A, KIAA1718
Uniprot	A0JNV9

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Yang, C., et al. Cell Res. 2010. 20: 886-898. 2. Osawa, T. et al. Proc. Nat. Acad. Sci. (USA). 2011. 108(51): 20725-20729.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200398	50419	20 ug	$495		Add to Cart

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